Fig. 5

GATA2 transcriptionally activates GATA2-AS1 expression. A RT-qPCR verified gene overexpression efficiency of GATA2 in CRC cells. B RT-qPCR was used to detect GATA2-AS1 expression in GATA2-overexpressing cells. C, D JASPAR database showed two potential GATA2-binding sites on GATA2-AS1 promoter. E, F Luciferase reporter assays detected the luciferase activity of GATA2-AS1 promoter at Full length, Site 1-MUT, Site 2-MUT and Site 1 + 2-MUT in CRC cells transfected with pcDNA3.1/GATA2 or shRNAs targeting GATA2. G, H ChIP assays detected the binding between GATA2-AS1 promoter and GATA2. ^*P < 0.05, ^**P < 0.01, n.s.: no significance