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Fig. 4 | Journal of Translational Medicine

Fig. 4

From: A feedback loop between GATA2-AS1 and GATA2 promotes colorectal cancer cell proliferation, invasion, epithelial-mesenchymal transition and stemness via recruiting DDX3X

Fig. 4

GATA2-AS1 recruits DDX3X to control the stability of GATA2 mRNA. A, B The cytoplasmic distribution of GATA2-AS1 in CRC cells was verified by subcellular fractionation and FISH assays. C ActD assays detected the stability of GATA2 in GATA2-AS1-shRNAs transfected SW480 and HCT116 cells. D, E RNA pull down assay showed the physical interaction between GATA2-AS1 and DDX3X. F The enrichment of GATA2-AS1 in Anti-DDX3X groups was detected by RIP assays. G, H RNA pull down and RIP assays detected the binding between GATA2 and DDX3X. I GATA2 RNA and protein levels were detected in DDX3X-ablated CRC cells. J ActD assays detected the stability of GATA2 in DDX3X-shRNAs transfected CRC cells. **P < 0.01

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