Fig. 5
From: Rhein attenuates angiotensin II-induced cardiac remodeling by modulating AMPK–FGF23 signaling
FGF23 overexpression obviously weakened the protective effects of rhein in vitro. A Representative micrographs of CMs stained with α-actinin (red) and DAPI (blue) (scale bars = 20 μm; n ≥ 50 cells per group). B Immunofluorescence staining of α-SMA expression (green) and nuclei (DAPI, blue) (n = 5 per group, ≥ 20 fields per group; scale bar = 100 μm). C Immunofluorescence DCFH-DA staining of ROS levels in CMs (n = 5 per group). D Immunofluorescence DHE staining of ROS levels in CFs (n = 5 per group). E CCK-8 quantification of CF proliferation (n = 5 per experiment). F, G, RT-qPCR quantification of ANP, BNP, collagen I, and TGF-β1 mRNA levels (n = 5 per experiment). *P < 0.05 and **P < 0.01 vs. the Ang II + LV-GFP group; #P < 0.05 and ##P < 0.01 vs, the Ang II + rhein25 + LV-GFP group