Fig. 4

RNA sequencing emphasized the biological processes and target molecules regulated to attenuate cardiac remodeling by rhein. A Heat map showing the expression patterns of differentially expressed genes. B KEGG analysis. C, D RNA sequencing results were validated by qPCR (n = 5 per group) and enzyme-linked immunosorbent assay (n = 5 per group). E Western blot analysis and quantification of FGF23 expression in cardiac tissue (n = 4 per group). F Western blot analysis and quantification of FGF23 expression in CMs or CFs (n = 4 per group). C, D **P < 0.01 vs. the control group; ^#P < 0.05 vs. the Ang II group. E **P < 0.01 vs. the sham group; ^#P < 0.05 and ^##P < 0.01 vs. the Ang II group