Fig. 3

Vascular changes in healing wounds. A Perfusion as measured by Laser doppler in wound bed over duration of healing. DFO demonstrates elevated perfusion measures throughout course of healing more closely mimicking nonirradiated wounds relative to radiated controls. B Laser Doppler perfusion measures reveal similar perfusion estimates between DFO and nonirradiated wounds when healed (mean: 196.8 vs. 174.9 respectively; p > 0.05). DFO wounds have significantly higher perfusion than vehicle (123.5;***p < 0.001) and IR untreated wounds (130.7;***p < 0.001). Representative images of Doppler scans of healed wounds are shown. C CD31 immunofluorescence reveals higher staining of CD31 with DFO (mean 407.4) relative to vehicle (208.0; ***p < 0.001) and IR untreated wounds (179.8; ***p < 0.001), with levels similar to nonirradiated wounds (378.1;p > 0.05). Representative images of CD31 staining on healed wound sections at 20 × magnification depicted per condition. Scale bar = 50 μm. DFO: deferoxamine; IR: irradiation; ns: not significant; POD: post-operative day