Fig. 6

CD4⁺ T cells and DP CD4⁺CD8⁺ T cells in mouse intestinal tissue detected by FCM and IF. Colonic tissues of five pairs of mice were used for FCM and IF to detect the population of CD4⁺ T cells and DP CD4⁺ CD8⁺ T cells. A–C FCM showed that the population of CD4⁺T cells was increased, while the population of DP CD4⁺CD8⁺ T cells was decreased significantly in the DSS group compared with the NC group. D–F IF showed that the population of CD4⁺CD8⁺ T cells was significantly decreased, whereas the population of CD4⁺ T cells was increased dramatically in the DSS group compared with the NC group (Nucleus emitted blue light, CD8⁺ T cells emitted green light, while CD4⁺ T cells emitted red light). Data are representative FCM and IF images (scale bar: 20 μm) or expressed as the mean ± SEM of each group. *P < 0.05, ***P < 0.001. NC normal control group, DSS DSS-induced colitis group