Fig. 5

Expression and methylation of Zbtb7b in DSS-induced colitis mice and control mice. Colonic tissues of five control mice and seven colitis mice were used for WB and qRT-PCR, five pairs of paraffin-embedded colonic tissues were used for IHC analysis, while colonic tissues of three control mice and five colitis mice were used for MSP. A, B WB and relative gray analysis of ZBTB7B protein in colonic tissue of mice. C Relative mRNA expression of Zbtb7b in colonic tissue of mice detected by qRT-PCR. D, E IHC and relative expression score of ZBTB7B protein in colonic tissue of mice. F, G Methylation of Zbtb7b in colonic tissue of mice detected by MSP. Data are representative electrophoretic bands or expressed as the mean ± SEM of each group.*P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001. NC normal control group, DSS DSS-induced colitis group, M methylated, U unmethylated