Fig. 5

Treatment with eFT-508 inhibits lung metastasis. Lung tissues harvested from animals in the different experimental groups described in Fig. 4 were processed for H&E and IHC (P-eIF4E) staining. Shown are representative H&E staining at indicated magnifications (A–D) and IHC staining (E–G) from vehicle (A, E), Adriamycin (B, F), eFT-508 (C, G), and adriamycin + eFT-508 (D, H) experimental groups. Robust lung micrometastatic colonies were visible both in the vehicle (A) and Adriamycin (B) experimental groups. Lung metastasis was less in the eFT-508 group (C). Combination therapy with adriamycin and eFT-508 almost completely attenuated lung metastasis (D). Scale bar, 100 µm