Fig. 6

RAF1 regulates tumor growth and lymphatic metastasis in vivo. A Fluorescence imaging of nude mice, red arrow indicates suspected lymphatic metastasis. B, C Luminescence area and photon flux analysis. D Anatomy of foot-pad primary tumors and metastatic lymph nodes in nude mice, red arrow indicates metastatic lymph nodes; E Measurements of primary tumors and lymph nodes volume. F–H qRT-PCR and WB assay of RAF1 and lymphangiogenic cytokines. I Immunohistochemical staining for RAF1 and LYVE-1. Abbreviations: sh-RNA, short hairpin RNA; NC, negative control; lv, lentivirus; OE, overexpression. *P < 0.05, **P < 0.01, ***P < 0.001