Fig. 11

LAGE1 and Sorafenib regulate tumor growth and lymphatic metastasis in vivo. A Fluorescence imaging of nude mice. B, C Luminescence area and photon flux analysis. D Anatomy of foot-pad primary tumors and metastatic lymph nodes in nude mice. E Measurements of primary tumors and lymph nodes volume. F–H qRT-PCR and WB assay of LAGE1 and lymphangiogenic cytokines. I Immunohistochemical staining for LAGE1 and LYVE-1. Abbreviations: sh-RNA, short hairpin RNA; NC, negative control; (+), addition Sorafinib; (−) without Sorafinib. *P < 0.05, **P < 0.01, ***P < 0.001