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Fig. 4 | Journal of Translational Medicine

Fig. 4

From: Stress-induced epinephrine promotes epithelial-to-mesenchymal transition and stemness of CRC through the CEBPB/TRIM2/P53 axis

Fig. 4

Chronic stress downregulates the stability of the p53 protein by promoting p53 ubiquitination. A-B HCT116 and LoVo cells were treated with EPI followed by treatment with cycloheximide (CHX) for the indicated times. The intensity of p53 expression at each time point was quantified by densitometry and plotted against time. C-D Immunoblots of HCT116 and LoVo cells treated with Epi for 5 days and then incubated with or without MG132 for 6 h. E Ubiquitin assays of HCT116 and LoVo cells treated with Epi and MG132. F-G Immunoblot analysis revealed that P53 overexpression reverses the effects of EPI-induced promotion of EMT and CSCs in CRC cells. H–J Mammosphere culture analysis revealed that p53 overexpression reverses the effect of EPI on the promotion of CSCs in CRC cells (scale bar: 100 μm). K–N Transwell assays revealed that p53 overexpression reverses the effect of EPI on the promotion of migration in CRC cells. O-P Colony formation assays revealed that p53 overexpression reverses the effect of EPI on the promotion of proliferation in CRC cells. *P < 0.05, **P < 0.01, ***P < 0.001

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