Fig. 7
From: Metformin counteracts stimulatory effects induced by insulin in primary breast cancer cells
Insulin-stimulated CXCL12/CXCR4 signaling triggers matrigel evasion of BCAHC-1 cells. A Representative pictures from the matrigel drops evasion assay in BCAHC-1 cells treated for 8 h with vehicle or 10 nM insulin alone or in combination with 10 μM CXCR4 antagonist AMD3100 or 2 mM metformin that was added to culture medium 18 h before the treatments. Cells were then cultured for additional 3 days to conditioned medium (CM) collected from CAFs, which were previously treated for 8 h with vehicle or 10 nM insulin, as indicated. Arrows point to the matrigel drop border. B Percentage of cells around the drop upon 3 days treatment from three independent experiments performed in triplicate. Scale bar: 500 μm. (*) indicates significant differences with respect to BCAHC-1 (+vehicle) exposed to CM/CAFs (+vehicle) sample (p < 0.05); (black square) indicates significant differences with respect to BCAHC-1 (+Insulin) exposed to CM/CAFs (+vehicle) sample (p < 0.05); (black circle) indicates significant differences with respect to BCAHC-1 (+vehicle) exposed to CM/CAFs (+Insulin) sample (p < 0.05); (white circle) indicates significant differences with respect to BCAHC-1 (+Insulin) exposed to CM/CAFs (+Insulin) sample (p < 0.05)