Fig. 3

JAML overexpression promotes migration and invasion in LUAD cells. a–d The wound healing assay indicated that JAML knockdown suppressed the migratory capabilities of A549 (a) and H1299 cells (b), and that JAML overexpression promoted the migratory capabilities of PC9 (c) and H1975 cells (d). Representative images of wound healing assays were obtained (left panel). ImageJ software was used to measure the migration rate (right panel). n = 3, unpaired t test, *P < 0.05, **P < 0.01, ***P < 0.001, compared with the SiNC or vector groups. e–h The transwell migration assay confirmed that JAML knockdown suppressed the migratory capabilities of A549 e and H1299 cells (f), and that JAML overexpression promoted the migratory capabilities of PC9 (g) and H1975 cells h. Representative images of transwell migration assays were obtained (left panel). ImageJ software was used to count the number of migrated cells (right panel). n = 3, unpaired t test, ***P < 0.01, compared with the SiNC or vector groups. i–l Transwell invasion assays indicated that the invasion ability was downregulated in A549 (i) and H1299 (j) cells when JAML was knocked down, and that the invasion ability was upregulated in PC9 (k) and H1975 cells (l). Representative images of transwell invasion were obtained (left panel). ImageJ software was used to count the number of invaded cells from 3 randomly selected fields (right panel). n = 3, unpaired t test, ***P < 0.001, compared with the SiNC or vector groups