Fig. 2

JAML overexpression promotes LUAD cell proliferation. a–d The efficiency of JAML knockdown in A549 (a) and H1299 cells (b) or overexpression in PC9 (c) and H1975 cells (d) were confirmed by Western blotting. The internal reference gene was β-actin. e–h Cells growth of A549 (e), H1299 (f), PC9 (g) and H1975 cells (h) with JAML knockdown or overexpression was analyzed with CCK8 assay. *P < 0.5, **P < 0.01, ***P < 0.001, compared with SiNC or Vector group. i–l Cell proliferation in A549 (i) and H1299 cells (j) after JAML knockdown and in PC9 (k) and H1975 cells (l) after JAML overexpression was observed through EdU assay, quantitative analysis n = 3, unpaired t test, **P < 0.01, ***P < 0.001, compared with SiNC or Vector group. m, n The colony formation showed that cell growth potential was inhibited in A549 cells (m) following JAML knockdown, and that cell growth potential was increased in PC9 cells (n) following JAML overexpression (top panels). The number of foci was shown in the bottom panels. Quantitative analysis n = 3, unpaired t test, *P < 0.05, compared with the ShNC group, **P < 0.01, compared with the vector group