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Table 5 Summary of the dPCR-NIPT assay results

From: A dPCR-NIPT assay for detections of trisomies 21, 18 and 13 in a single-tube reaction-could it replace serum biochemical tests as a primary maternal plasma screening tool?

  

True aneuploidy statuses

dPCR-NIPT assay performance

 

 + 

−

Sens (%)

Spec (%)

dPCR-NIPT test results

T21a

 + 

25

7

100.00

97.28

−

0

250

T18a

 + 

9

1

90.0

99.63

−

1

271

T13

 + 

1

5

100.00%

98.22

−

0

276

dPCR-NIPT combined

Screening results*

 + 

36

12

100.00

95.12

−

0

234

  1. The test results of the dPCR-NIPT assay were compared to true aneuploid statuses for these samples. The top table with a clean background evaluated the dPCR-NIPT assay's T21, T18 and T13 performances, separately. The bottom table with a grey background demonstrated the dPCR-NIPT assay's overall performance as a screen tool by combining all the T21, T18 and T13 results. Sens sensitivity, Spec specificity
  2. *One T18 positive sample was called as T21 by the dPCR-NIPT assay, this result was counted as T21 false positive and T18 false negative, separately, for the dPCR-NIPT assay's T21 and T18 evaluations. However, when the dPCR-NIPT assay acted as an intact test for screening aneuploid pregnancies, this T18 positive sample should be counted as a screen positive for trisomy