Fig. 4

CCL20 may induce crizotinib resistance by activation of angiogenesis via JAK2/STAT3-CCL20-VEGFA/IL6 axis. A a) Tube formation of HUVECs cultured in H3122 culture medium (CM), H3122CR CM, H3122 CM supplemented with human recombinant CCL20, and H3122 CM supplemented with rhCCL24. b) Tube formation of HUVECs cultured in the CM of H3122CR transfected with si-negative control (si-NC), si-CCL20, and si-CCL24. The number of tubes and capillary length were analyzed to evaluate angiogenic activity of CCL20 and CCL24; B, C Knockdown of CCL20 suppressed the protein expression of CCL2, IL6, and VEGFA in H3122CR compared with si-NC (Control); D STAT3 was upregulated in H3122CR compared with H3122; E, F Stattic inhibited the JAK2/STAT3 pathway and the protein expression of CCL20 and VEGFA in H3122CR. GAPDH served as a loading control. *p < 0.05, **p < 0.01, ***p < 0.001, ns not significant