Fig. 6

DLEU1 expression is epigenetically regulated in ESCC. A Scatter plots presenting the negative correlation between DLEU1 expression levels and its promoter methylation values in the TCGA-ESCC dataset. B Kaplan–Meier curves of overall survival (left) and disease-specific survival according to DLEU1 DNA methylation in ESCC patients from the TCGA project. C Relative DLEU1 expression levels in a panel of ESCC cell lines cultured in the presence or absence of decitabine (0.5 μM) for 48 h. D Immunoblotting analysis of the indicated proteins in EC109 and KYSE-410 cells treated with decitabine. β-actin was used as a loading control