Fig. 5

The DLEU1/DYNLL1 axis modulates mitochondrial apoptotic signaling. A DYNLL1 was successfully knocked down in KYSE-150-DLEU1 cells. β-actin was used as a loading control. B Immunoblotting analysis of BIM and BCL2 expression after DYNLL1 depletion in KYSE-150-DLEU1 cells. C Immunoblotting analysis of BIM and BCL2 expression after DLEU1 overexpression in KYSE-150 cells. D Cells transfected with wild-type or DYNLL1 mutants were subjected to immunoblotting analysis with the indicated antibodies. E The effects of concomitant silencing of DYNLL1 and BIM on BCL2 expression were evaluated by immunoblotting in KYSE-150 cells with ectopic DLEU1 expression. F Annexin V/PI flow cytometry analysis of the effects of DYNLL1 and BIM depletion on cisplatin-induced cell apoptosis in DLEU1-overexpressing KYSE-150 cells. Significance was assessed by one-way ANOVA with Tukey’s post-hoc test. *P < 0.05, **P < 0.01. G Representative images (left) and scatter plot (right) showing a positive correlation among DLEU1, DYNLL1, and BCL2 in clinical ESCC specimens (n = 38). Scale bar, 50 μm. H Representative immunohistochemical staining of DYNLL1 and BCL2 proteins in DLEU1-overexpressing ESCC xenografts. Scale bar, 50 μm