Fig. 2

DLEU1 promotes ESCC growth by inhibiting cell apoptosis. A The effects of DLEU1 knockdown by siRNA on cell growth were measured by CCK-8 assay. B Effects of DLEU1 knockdown on colony formation in EC109 and KYSE-410 cells. C Representative images and quantification of DLEU1-silenced KYSE-410 cells that migrated (left) or invaded (right) through the Transwell membrane. Scale bar, 50 μm. D The effects of DLEU1 overexpression on cell growth were measured by CCK-8 assay. E Representative images and quantification of migratory (left) or invasive (right) DLEU1-overexpressing KYSE-150 cells. Scale bar, 50 μm. F Effect of DLEU1 on xenograft tumor growth in nude mice. Tumor volume was measured every other day and calculated. G Tumors were excised from mice at the end of the experiment. H Tumor weights were measured. I, J Annexin V/PI flow cytometry analysis of the effects of DLEU1 on cisplatin-induced cell apoptosis in DLEU1-silenced KYSE-410 (I) or DLEU1-overexpressing KYSE-150 cells (J). K, L Representative images showing apoptotic ESCC cells measured by JC-1 (K) and TUNEL staining (L). Scale bar, 50 μm. Significance was determined by two-way ANOVA with Sidak's post-hoc test (A, F), one-way ANOVA with Tukey's post-hoc test (B, C, I), or unpaired Student's t test (E, F, H, J). *P < 0.05, **P < 0.01, ***P < 0.001