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Fig. 2 | Journal of Translational Medicine

Fig. 2

From: Platinum-induced mitochondrial OXPHOS contributes to cancer stem cell enrichment in ovarian cancer

Fig. 2

Expression of genes involved in mitochondrial OXPHOS increases after cisplatin treatment. Gene expression of the indicated genes by RT-qPCR in OVCAR5 (A) OVCAR3 (B) and OVSAHO (C) cells untreated or treated with 12 µM, 15 µM or 4 µM cisplatin, respectively, for 16 h. Expression of HIF-1α in OVCAR5 (D) and OVCAR3 (E) cells treated as in A and C. F OVCAR5 cells were untreated (U) or treated with 12 µM cisplatin for 16 h (T). Cell lysates were collected and analyzed for indicated proteins by western blot. G c-Myc expression in untreated (U) or 12 µM cisplatin treated (T) OVCAR5 cells. Expression of the indicated glycolysis genes in OVCAR5 (H), OVCAR3 (I) and OVSAHO cells (J) treated as in A, B and C, respectively. Graphs display mean fold change ± SEM relative to untreated. Expression of all the genes was normalized to the house keeping gene Actin B. For all untreated versus cisplatin treated, P values *< 0.05, **< 0.005 and ***< 0.0005

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