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Fig. 5 | Journal of Translational Medicine

Fig. 5

From: C2orf40 inhibits metastasis and regulates chemo-resistance and radio-resistance of nasopharyngeal carcinoma cells by influencing cell cycle and activating the PI3K/AKT/mTOR signaling pathway

Fig. 5

C2orf40 overexpression in NPC cells could induce the cell cycle arrest at G2/M phase. A GO and KEGG pathway enrichment analyses of 544 genes that were negatively correlated with C2orf40 expression (|R|> 0.3, P < 0.05) using Metascape. Data were imported from the three GEO datasets (GSE12452, GSE53819, and GSE64634). B‒C A strong inverse correlation of C2orf40 expression levels with the expression levels of both CCNE1 (B) and CDK1 (C) was noted in the three GEO datasets. D‒E The representative images (D) and statistical analysis (E) of Western blotting of the expression levels of cell cycle-related genes (CDK1, p-CDK1, p-Rb, Rb, CCNE1 and CCNB1) in HONE-1 and SUNE-1 cells. GAPDH was used as an internal reference. F Cell cycle analyses carried out by flow cytometry of HONE-1 and SUNE-1 cells showed a cell cycle arrest at G2/M phase after transiently transfecting C2orf40 plasmids. The experiments were performed in triplicate and repeated twice. Data are presented as the mean ± SD. *P < 0.05, **P < 0.01, ***P < 0.001

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