Fig. 3

Overexpression of C2orf40 enhances chemo-sensitivity of NPC cells to cisplatin in vitro. A‒B The endogenous expression level of C2orf40 was determined by Western blotting (A) and qRT-PCR (B) after stably overexpressing C2orf40 in SUNE-1 and HONE-1 cells. C SUNE-1 and HONE-1 cells were pretreated with cisplatin for the indicated concentrations, and then, they were subjected to CCK-8 assay. D–F SUNE-1 and HONE-1 cells were exposed to 1 µg/ml cisplatin for 48 h. D The sensitivity of NPC cells to cisplatin was evaluated using colony formation assay. E The apoptosis level of NPC cells was assessed using TUNEL staining. The results were shown as a percentage of TUNEL + cells versus total cells. F Western blot analysis of the expression levels of apoptosis-related genes (C-caspase-3, C-PARP, and Bax) in NPC cells. The β-actin protein was used as loading control. The experiments were performed in triplicate and repeated twice. Data are presented as the mean ± SD. *P < 0.05, **P < 0.01, ***P < 0.001