Fig. 3

Shared clonotypes with TILs were expanded by co-culture with tumor cells. a TCRβ clonotypes of lymphocytes before, 1 and 2 weeks after co-cultured with tumor-cells were compared with those of 4 TIL fragments which were recognized over 0.1% in at least 1 TIL fragment of C165. The number indicates the % of the frequency of shared clonotypes detected in each lymph node. b TCR clonotypes of lymphocytes before and after co-cultured with tumor-cells were compared with those of TILs which occupied over 0.01% in total T cells of C207. The number indicates the % of the frequency of shared clonotypes detected in each lymph node. c Lymphocytes of non-metastatic lymph nodes of C207 were stained with anti-TCRαβ, CD4, CD8α and PD-1 antibodies. CD4 and CD8 T cells were sorted by PD1 expression pattern. TCRβ clonotypes of TILs were compared with each population in each lymph nodes. The number indicates the frequency of shared clones. d TCRβ clonotypes of lymphocytes that occupied more than 0.1% of CD8 T cells in each lymph nodes were compared between lymph nodes by PD1 expression pattern. The number indicates the % of the frequency of shared clonotypes between each sample