Fig. 1From: Serum proteome alterations during conventional and extracorporeal resuscitation in pigsSchematic experimental design and proteomics workflow. Pigs underwent a successive series of treatments including cardiac arrest (CA), cardiopulmonary resuscitation (CPR), basic life support (BLS), advanced life support (ALS) and extracorporeal cardiopulmonary resuscitation (ECPR) using CARL (controlled automated reperfusion of the whole body). Blood sampling was performed at the three marked time points including “Baseline” (time point 1), “after ALS” (time point 2) and “after CARL” (time point 3). Subsequent proteomic sample processing involved depletion of albumin and immunoglobulin G (IgG), proteolytic digest and peptide clean-up before samples were subjected to liquid chromatography–tandem mass spectrometry (LC–MS/MS) measurement. VF, ventricular fibrillationBack to article page