Fig. 2
From: mTOR pathway gene mutations predict response to immune checkpoint inhibitors in multiple cancers
Identification of mTOR pathway genes and two-stage survival analysis in cancer patients with ICI treatment. In discovery stage of 1661 ICI-treated cancer patients from TMB and immunotherapy study by MSKCC, we performed stepwise Cox regression with both direction of backward and forward using 23 mTOR pathway genes, which further yielded 8 genes upon iteration, including FGFR2, PIK3C3, FGFR4, FGFR1, FGF3, AKT1, mTOR, and RPTOR (A). The AIC and variables left for each step during stepwise Cox regression (B). The biological role of the 8 genes, which were marked red, in mTOR pathway (C). Mutation states of the 8-gene signature for the 1661 ICI treated patients were used for integrated survival comparison by Kaplan–Meier curves and multivariate Cox regression with adjustment for age, gender, treatment and TMB (D). The survival association was also tested in 2244 patients without ICI treatment (E). Using RMST analysis, the survival association were validated in 553 ICI-treated patients from other 6 independent cohorts and 763 patients without ICI treatment from TCGA (F, G)