Fig. 4

Necroptosis phenotype-related DEGs in colorectal cancer. A Consensus clustering of TCGA tumor samples using necroptosis phenotype-related signature. Clinical and molecular characteristics are shown on the top. The difference of three gene clusters was compared through the Pearson’s Chi-square test. B Kaplan–Meier curves for overall survival of three gene clusters in TCGA. The P value was calculated by the log-rank test. C Gene ontology enrichment (GO) analysis of genes A and genes C. D Tumor purity, immune and stromal score of three gene clusters in TCGA cohort. The statistical difference of three clusters was compared through the Kruskal–Wallis H test. *P < 0.05; **P < 0.01; ***P < 0.001. E Comparison of PD-L1 and PDCD1 (PD-1) expression between three gene clusters. The difference of three clusters was compared through the Wilcox test. *P < 0.05; **P < 0.01; ***P < 0.001. F Barplot shows the ssGSEA score of immune cell subtypes from the study of Charoentong in three gene clusters. The statistical difference of three clusters was compared through the Kruskal–Wallis H test. *P < 0.05; **P < 0.01; ***P < 0.001