Fig. 3

Distinct tumor microenvironment infiltration in necroptosis-related clusters. A–C Alluvial diagram of clusters in groups with different molecular subtypes. D Relative abundance of 22 tumor-infiltrating immune cells (TIICs) of three clusters in TCGA cohort. The statistical difference of three clusters was compared through the Kruskal–Wallis H test. *P < 0.05; **P < 0.01; ***P < 0.001. E Barplot shows the ssGSEA score of immune cell subtypes from the study of Charoentong in three necroptosis-related clusters. The statistical difference of three clusters was compared through the Kruskal–Wallis H test. *P < 0.05; **P < 0.01; ***P < 0.001. F Tumor purity, immune and stromal score of three NRCs in TCGA cohort. The statistical difference of three clusters was compared through the Kruskal–Wallis H test. *P < 0.05; **P < 0.01; ***P < 0.001. G Comparison of PD-L1 and PDCD1 (PD-1) expression between three NRCs. The difference of three clusters was compared through the Wilcox test. *P < 0.05; **P < 0.01; ***P < 0.001