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Fig. 3 | Journal of Translational Medicine

Fig. 3

From: Single-cell landscape of immunocytes in patients with extrahepatic cholangiocarcinoma

Fig. 3

CD8 + T cell heterogeneity in ECCA. A UMAP plot of CD8 + T cells from ECCA patients, with each cell color coded by cell subcluster according to gene expression characteristics. B UMAP plot of the CD8+ T cell subclusters annotated by marker genes. C The heatmap shows the normalized mean expression of differentially expressed genes (rows) associated with the selected CD8 + T cell functions in each CD8 + T cell subcluster. D UMAP plot of color-coded CD8 + T cells from the tumor tissues, paratumor tissues and peripheral blood of ECCA patients. E The proportion of eight CD8 + T cell subclusters in peripheral blood, paratumor, and tumor tissues from ECCA patients. F Volcano plot showing differentially expressed genes in CD8 + Tex cells. Each red square denotes a highly expressed gene that met the p value and fold difference criteria. G Violin plots showing the normalized mean expression of differentially expressed genes associated with the selected CD8 + T cell functions across CD8 + Tn (C7-CD8), CD8 + Teff (C2-CD8, C3-CD8 and C8-CD8) and CD8 + Tex (C1-CD8 and C4-CD8) cells. H The ordering of CD8 + T cells along pseudotime in a two-dimensional state space defined by Monocle2. Each point corresponds to a single cell, and each color represents a CD8 + T cell subcluster

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