Fig. 5

miR-18a-5p targeted and inhibited NACC1 expression. A Chromosome expression maps of significantly differentially expressed genes in OC included in TCGA and GTEx (green and red lines: evidently differentially expressed genes; position: the location in chromosome; red: upregulated genes; green: downregulated genes); B Prediction of target genes of miR-18a-5p: 3 circles respectively represented the prediction results of Starbase database, TargetScan databases and significantly upregulated genes in OC, and the middle part indicated the intersection of the 3 groups of data; C NACC1 expression in OC and normal samples included in TCGA and GTEx (abscissa: sample type; ordinate: expression level; red: tumor sample; gray: normal sample); D Expression of NACC1 mRNA in 76 early/advanced OC tissues and 36 benign ovarian tumor tissues was measured by RT-qPCR; E Correlation analysis of miR-18a-5p and NACC1; F Binding sites of miR-18a-5p and NACC1 was predicted via Starbase database; G Targeted binding of miR-18a-5p and NACC1 was validated using dual-luciferase assay; H Expression of NACC1 mRNA in CAOV3/ES2 cells and normal ovarian epithelial cells (IOSE80) was detected by RT-qPCR; I, NACC1 expression in OC cells was determined by WB. Cell experiments were repeated 3 times. Data were exhibited as mean ± SD. One-way ANOVA was used for comparisons between groups and Tukey's multiple comparisons test was employed for the post hoc test. *P < 0.05, **P < 0.01, ***P < 0.001