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Fig. 1 | Journal of Translational Medicine

Fig. 1

From: miR-18a-5p derived from mesenchymal stem cells-extracellular vesicles inhibits ovarian cancer cell proliferation, migration, invasion, and chemotherapy resistance

Fig. 1

Identification of hMSCs and EVs. The hMSCs were passage-cultured. A Morphology of the fourth-passage hMSCs was observed under a microscope; B Expression of specific molecular markers CD29, CD34, CD45, CD73, CD90, CDl05, CD117 and HLA-DR was detected using flow cytometry; C Osteogenic differentiation was observed by Alizarin red staining; D Adipogenic differentiation was observed via Oil Red O staining; E Cartilage differentiation was observed through Alcian blue; F Morphology of hMSC-EVs was observed using TEM after the extraction of EVs; G Size distribution of EVs was analyzed by NTA; H Expression of CD63, CD9, CD81, HSP70 and Calnexin in EVs was measured using WB

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Journal of Translational Medicine

ISSN: 1479-5876

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