Fig. 3

Skeletal muscle myopathy in 4-month-old Hspa4-KO mice. A Representative sections from tibialis anterior (TA), soleus and gastrocnemius (GC) muscles, stained with H&E showing centrally nucleated myofibers (white arrows). B and C Fiber size distribution in tibialis anterior (n = 4 mice/genotype; 250 fibers/ mouse) B, and average percentage of myofiber with central nucleus C. D–F Quantitative real time PCR analysis of inflammatory markers D, Myh3 E and Myh8 F expression. Data are expressed as mean ± SEM. *p < 0.05, **p < 0.01, ***p < 0.001 vs. WT controls, two-tailed unpaired Student's t-test. Numbers within columns indicate mice