Fig. 6

Upregulated miR-758-5p promoted osteogenic and odontogenic differentiation. A Transfection efficacy was shown by qRT-PCR in the manner of relative miRNA expression in PDLSCs. B CCK-8 assay showed the impact of overexpression of miR-758-5p on proliferation. C The relative expression of ALP, DSPP, OSX, and RUNX2 in the mimic, mimic NC, inhibitor, and inhibitor NC group. D Western blot assay detected the expression of ALP, DSPP, RUNX2, and OSX when miR-758-5p was overexpressed or inhibited. E Statistic analysis of the grayscale of western blot and data were shown as the ratio of the target protein to GAPDH. F Immunofluorescence photos showed that the intensity (represented the expression of ALP) in mimic, mimic NC, inhibitor, and inhibitor NC groups. G Result of ALP staining. H Result of ARS staining. I Quantification of ARS staining. J qRT-PCR showed that miR-758-5p regulates LMBR1. K Western blot showed that miR-758-5p regulates LMBR1. n = 3, **P < 0.01, *P < 0.05. Scale bar, 100 μm