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Fig. 3 | Journal of Translational Medicine

Fig. 3

From: Extracellular vesicles from the inflammatory microenvironment regulate the osteogenic and odontogenic differentiation of periodontal ligament stem cells by miR-758-5p/LMBR1/BMP2/4 axis

Fig. 3

Isolation and identification of iDPSC-EV. A Results of CCK-8 assay. B Results of Flow cytometry analysis of cell cycle. C Relative mRNA expression of DSPP, OSX, and RUNX2 was demonstrated by qRT-PCR. D The protein levels of DSPP, OSX, and RUNX2 was determined by western blot. E The quantification of band intensities was shown by a histogram with the manner in the ratio of the target protein to GAPDH. F NTA showed the size distributions of these EV. G TEM photos of EVs, scale bar, 100 μm. H The expression of CD9, CD63 and CALNEXIN in EV and parent cells. I The result of EV phagocytosis, scale bar, 100 μm. n = 3, **P < 0.01, *P < 0.05

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