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Fig. 4 | Journal of Translational Medicine

Fig. 4

From: Effects of preoperative high-oral protein loading on short- and long-term renal outcomes following cardiac surgery: a cohort study

Fig. 4

Effect of dietary protein dose response treatment of primary human kidney tubule cells on the presence of TIMP-2 and IGFBP7 in the apical conditioned media. Isolated primary human proximal and distal tubule cells were subjected to dose–response analysis with whey protein, and secretion of the AKI biomarkers IGFBP7 and TIMP2 was assessed by immunoblot analysis of apical conditioned media. Densitometry values for (A) IGFBP7 from proximal tubule cells (4 genetically different samples) and (B) TIMP-2 from distal tubule cells (3 genetically different samples) were adjusted to protein concentration and normalized to no treatment. Asterisks indicate statistical significance from no treatment (p < 0.05). AKI, acute kidney injury; IGFBP7, insulin-like growth factor binding protein 7; TIMP-2, tissue inhibitor of metalloproteases-2

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