Fig. 4

SIRT1 prevented CSE-induced LF activation by regulating lipid metabolism in an autophagy-dependent pathway. A Level of SIRT1 was detected. B Effect of SIRT1 activator SRT1720 on the expression of Collagen I, α-SMA, CPT1a and PPARα. C Nile Red Staining. D, E Representative pictures of lipophagy as indicated by the co-localization of LC3 (D) or LTR (E) with Bodipy 493/503. F, G Western blot analysis for Collagen I, α-SMA, p62, LC3 I and LC3 II. H Representative pictures and numbers of autophagosomes (yellow) and autolysosomes (red). I Level of collagen I, α-SMA, CPT1a and PPARα were examined. *p < 0.05 versus control, ^#p < 0.05 versus CSE, ^&p < 0.05 versus CSE + SRT1720 (n = 3)