Table 3 Sequence of the primers used for verifying JAK-STAT pathway
From: Inhibition of NOS1 promotes the interferon response of melanoma cells
Gene name | Forward primer (5’–3’) | Reverse primer (5’–3’) |
|---|---|---|
GAPDH | CTCCAAAATCAAGTGGGGCG | TGGTTCACACCCATGACGAA |
IL13RA2 | AAAGTTCAGGATATGGATTGCGT | GAAGTACACCTATGCCAGGTTTC |
SPRY4 | TCTGACCAACGGCTCTTAGAC | GTGCCATAGTTGACCAGAGTC |
PIK3R3 | TACAATACGGTGTGGAGTATGGA | TCATTGGCTTAGGTGGCTTTG |
LIF | CCAACGTGACGGACTTCCC | TACACGACTATGCGGTACAGC |
IFNAR2 | TCATGGTGTATATCAGCCTCGT | AGTTGGTACAATGGAGTGGTTTT |
CCND1 | GCTGCGAAGTGGAAACCATC | CCTCCTTCTGCACACATTTGAA |
IL24 | CACACAGGCGGTTTCTGCTAT | TCCAACTGTTTGAATGCTCTCC |
EPOR | ACCTTGTGGTATCTGACTCTGG | GAGTAGGGGCCATCGGATAAG |
IRF9 | GCCCTACAAGGTGTATCAGTTG | TGCTGTCGCTTTGATGGTACT |
IFNGR2 | CTCCTCAGCACCCGAAGATTC | GCCGTGAACCATTTACTGTCG |
IFNAR1 | AACAGGAGCGATGAGTCTGTC | TGCGAAATGGTGTAAATGAGTCA |
JAK1 | CTTTGCCCTGTATGACGAGAAC | ACCTCATCCGGTAGTGGAGC |