Fig. 1
Effect of tanimilast on CD4+ T cell activation by LPS-moDCs. A moDCs were treated or not (-) with tanimilast (TAN) or budesonide (BUD) (both at 10−7 M) for 1 h before stimulation with LPS. After 24 h, moDCs were collected and co-cultured with CFSE-stained allogenic CD4+ T cells for 6 days. Alloreactive T cell proliferation was assessed by measuring CellTrace-CFSE dye loss by flow cytometry. Left, dot plot from one representative experiment (1:20 ratio). Right, line graph from three independent experiments with the indicated DC:T cell ratio. Data are expressed as mean ± SEM (n = 3) of the percentage of proliferating CD4+ T cells. B moDCs treated as described in A were incubated with T cells for 6 days. Intracellular IFN-γ and IL-13 were evaluated by FACS analysis. Left, dot plot from one representative experiment (1:20 ratio). Right, bar graphs from four independent experiments. Data are expressed as mean ± SEM (n =  4) of single IFN-γ-(left Y axis) and IL-13-(right Y axis) producing T cells. A, B #P < 0.05 versus (-) and *P < 0.05 versus LPS by one-way ANOVA with Dunnett’s post-hoc test