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Fig. 5 | Journal of Translational Medicine

Fig. 5

From: SRSF9 promotes colorectal cancer progression via stabilizing DSN1 mRNA in an m6A-related manner

Fig. 5

DSN1 deficiency inhibits proliferation, migration and invasion by SRSF9-overexpressing CRC cells. A, B Western blotting was used to measure the protein levels of DSN1 in DSN1-deficient CRC cells upon overexpression of SRSF9. CE CCK-8 assays (C, D) and colony formation assays (E) were performed in the CRC cells described in A, B (**p < 0.01; ***p < 0.001; ****p < 0.0001, two-way ANOVA and Student’s t test). F Cell cycle distribution was analyzed by flow cytometry with CRC cells described in (A-B) (**p < 0.01; ***p < 0.001, one-way ANOVA). G, H Transwell assays were performed using CRC cells as described in A, B. Scale bar = 100 μm (*p < 0.05; **p < 0.01; NS: not significant, Student’s t test). I, J Wound healing assays were performed using CRC cells as described in A, B. Scale bar = 200 μm (*p < 0.05; **p < 0.01, Student’s t test). Each experiment was performed in triplicate independently

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