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Fig. 4 | Journal of Translational Medicine

Fig. 4

From: SRSF9 promotes colorectal cancer progression via stabilizing DSN1 mRNA in an m6A-related manner

Fig. 4

Identification of SRSF9 targets in colorectal cancer. A Volcano plots displaying DEGs (1238) between 17 pairs of CRC tissues and adjacent normal tissues from the GEO database (GSE32323) (log2(fold change) > 1, p < 0.05). B Volcano plots showing DEGs (1360) between 482 CRC tissues and 42 adjacent normal tissues from the TCGA database (log2(fold change) > 1, p < 0.05). C Venn diagram displaying the overlapping gene (DSN1). D DSN1 mRNA levels were determined based on TCGA. E qRT-PCR assays were used to measure the RNA levels of DSN1 in CRC cell lines and in an immortalized normal intestinal epithelial cell line. (*p < 0.05; **p < 0.01, Student’s t test). F, G qRT-PCR (F) and Western blot (G) assays were used to detect the expression of DSN1 in 16 paired fresh CRC tissues. (*p < 0.05; ****p < 0.0001, Student’s t test). H Survival analysis of CRC patients based on the GEO database (GSE17538) for the correlation between DSN1 expression and overall survival (p < 0.05, log-rank test). I The expression of SRSF9 and DSN1 was positively correlated in colon adenocarcinoma tumors in the GEPIA database. JM Western blot assay showing the protein level of DSN1 after SRSF9 overexpression (J, K) and knockdown (L, M) in CRC cells. (N, O) Representative IHC staining images of SRSF9- and DSN1-positive staining in xenografted tumors. Scale bar = 50 μm. PR Western blot assays (P, R) and qRT-PCR assays (Q) were performed to determine the correlation between the expression of DSN1 and that of SRSF9 in 16 pairs of fresh CRC tissues. Data are presented as the mean ± standard deviation of the values obtained in three independent experiments

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