Fig. 3

Inhibition of SRSF9 suppresses proliferation, migration and invasion by CRC cells in vitro and tumor growth in vivo. A, B The indicated infected cells were subjected to qRT-PCR (left) and Western blotting (right). C, D CCK-8 and colony formation assays were performed in SRSF9 knockdown CRC cells (**p < 0.01; ***p < 0.001, two-way ANOVA and Student’s t test). E Cell cycle distribution was analyzed by flow cytometry in SRSF9 knockdown CRC cells (*p < 0.05; ***p < 0.001, one-way ANOVA). F, G Transwell assays were performed using SRSF9 knockdown CRC cells. Scale bar = 100 μm (***p < 0.001, Student’s t test). H, I Wound healing assays were performed in SRSF9 knockdown CRC cells. Scale bar = 200 μm (**p < 0.01; ***p < 0.001, Student’s t test). J The indicated stable cells were subjected to Western blotting. K Tumor nodules were collected and used to evaluate the difference in growth that occurred due to the influence of SRSF9 knockdown. L Tumor growth curve based on tumor size measurements (****p < 0.0001, two-way ANOVA). Data are presented as the mean ± standard deviation of the values obtained in three independent experiments