Fig. 6

Overexpression of CYP2E1 inhibits EMT in HCC cells. A Representative images of the morphological changes associated with EMT of MHCC-97H-CYP2E1, SMMC-7721-CYP2E1 and the respective control cells (×100). B Protein levels of EMT markers (E-cadherin, vimentin and N-cadherin) as well as EMT-related transcription factors (Twist, Slug and Snail) of the indicated cells detected by western blot. C Typical images of immunofluorescent staining for E-cadherin and vimentin in MHCC-97H and SMMC-7721 cells (×100). D Proposed model of the tumor suppressor role of CYP2E1 in HCC. Overexpression of CYP2E1 induces ROS accumulation and promotes the ubiquitin-mediated degradation of Dvl2, thereby, inhibiting the Wnt/β-catenin signaling and playing a suppressive role in HCC