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Fig. 3 | Journal of Translational Medicine

Fig. 3

From: CYP2E1 plays a suppressive role in hepatocellular carcinoma by regulating Wnt/Dvl2/β-catenin signaling

Fig. 3

CYP2E1 inhibits Wnt/β-catenin signaling via reducing the Dvl2 protein level. A Luciferase activity of TOPFlash in MHCC-97H-CYP2E1, SMMC-7721-CYP2E1 and their respective control cells. Data are shown as mean ± sd (n = 3 in each assay). *P ≤ 0.05, **P ≤ 0.01 vs. control cells. B Protein level of Dvl2, β-catenin, TCF4, LEF1 and c-Myc (Wnt/β-catenin signaling-related molecules) in control and CYP2E1-overexpressing HCC cells measured by western blot. C The protein expression of CYP2E1, Dvl2, β-catenin, and c-Myc in control and CYP2E1-overexpressing HCC cells at 6 h after treatment with the CYP2E1 inhibitor CMZ (40 μM). D The OD450 value of MHCC-97H-CYP2E1, SMMC-7721-CYP2E1 and the respective control cells after treatment with the CYP2E1 inhibitor CMZ (40 μM). E Representative images and statistical graphs (right panel) of transwell assays for the indicated cells after treatment with the CYP2E1 inhibitor CMZ (40 μM) (×100). F Representative images and statistical graphs (right panel) of scratch wound assays of MHCC-97H-CYP2E1, SMMC-7721-CYP2E1 and the respective control cells after treatment with the CYP2E1 inhibitor CMZ (40 μM) (×100). Data in 3D, 3E and 3F are shown as mean ± sd (n = 3–6 in each assay). *P ≤ 0.05, **P ≤ 0.01, ***P ≤ 0.001 vs. CYP2E1

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