Fig. 2

Overexpression of CYP2E1 inhibits the proliferation, migration, and invasion of HCC cells in vitro and in vivo. A CYP2E1 protein expression was evaluated by western blotting in control and CYP2E1-overexpressing HCC cells. B The effect of CYP2E1 overexpression on cellular survival as determined by CCK-8 assays. C Representative images from scratch wound assays of MHCC-97H-CYP2E1 (left panel), SMMC-7721-CYP2E1 (middle panel), and the respective control cells (×100). Statistical graphs of scratch wound assays (right panel). D Representative images and statistical analysis (down panel) of transwell assays for MHCC-97H-CYP2E1, SMMC-7721-CYP2E1, and the respective control cells (×100). E Image and growth curve of xenograft tumors in nude mice injected with the indicated cells (n = 3 in each assay). F Images of the liver orthotopic transplantation tumors in nude mice implanted with the subcutaneous tumors from MHCC-97H-CYP2E1 or MHCC-97H-GFP cells. G Representative H & E images of lung tissue slides of nude mice injected by tail vein with MHCC-97H-CYP2E1 and MHCC-97H-GFP. The data in B–E are presented as the means ± sd. *P ≤ 0.05; **P ≤ 0.01; ***P ≤ 0.001 vs. control group