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Fig. 4 | Journal of Translational Medicine

Fig. 4

From: Single-cell N6-methyladenosine regulator patterns guide intercellular communication of tumor microenvironment that contribute to colorectal cancer progression and immunotherapy

Fig. 4

NMF clusters of m6A methylation regulators for T cells and B cells. A t-SNE plot for 23,115 T cells by 8 cell types in the SMC dataset, including CD + 4, CD + 8, Treg, NK, T helper 17, T follicular helper, gamma delta T, and Unknown. B Cell–Cell communications from main m6A-related T cells to epithelia cells by Cell chat analysis. (C1, HNRNPA2B1 dominant; C2, IGF2BP1 and HNRNPC dominant; C3, IFG2BP3, WTAP, and FTO dominant; C4, ALKBH5, YTHDF2, and YTHDC1 dominant). C Bar plot showing the number and percentage of methy-T cell clusters, including the cluster without m6A methylation regulator expression (n = 1265), among different cell types of T cells. D Heatmap showing significantly different TFs among m6A clusters in CD + 4, CD + 8, Treg, and NK cells by comparing their average AUC by using pySCENIC in Python (Kruskal–Wallis test, p < 0.001). TF activity is scored using AUCell. E Heatmap showing significantly different features among methy-T clusters in CD + 4, CD + 8, Treg and NK cells, including four T function signatures (T exhaustion score, T cytotoxic score, T effector score, and T evasion score), as well as some immune stimulators, inhibitors and T cell function marker genes (Kruskal–Wallis test, p < 0.001). F Cell–Cell communications between main m6A-related B cells types by Cell chat analysis. G, H Bar plot for the number and percentage of m6A clusters by using the NMF clustering algorithm for 9146 B cells as above. J Heatmap showing significantly different TFs among m6A clusters in total B cells

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