Fig. 7

The impact of POU2F1 and LINC01564 on GC metastasis in vivo. Analysis of an experimental metastatic animal model was performed by injecting SGC-7901 cells stably transfected with POU2F1 (OE), POU2F1 (OE) plus LINC01564 (KD), or empty vector into nude mice (n = 4). A Tumor tissues from the mice in each group were stained with H&E staining. The number and relative area of metastatic foci was shown and quantified. B Tumor tissues from the mice in each group were stained with anti-KI67 and anti-MMP9 antibodies in immunohistochemistry assay. C PCR assay was performed to detect the expression levels of LINC01564 and POU2F1 in the collected tumor tissues. Significant differences are indicated by *p < 0.05, **p < 0.01 and ***p < 0.001