Fig. 5

LINC01564 directly interacts with POU2F1 RNA. A RNA stability assay of POU2F1 and 18S in SGC-7901 (left) and MKN-45 (right) cells. A Represents NC. B Represents LINC01564 OE. C Represents LINC01564 KD. Cells were treated with 5 μg per ml actinomycin D and collected at 0, 1, 2, 4, 6, and 8 h. The subcellular localizations of LINC01564 and POU2F1 were visualized by RNA FISH in MKN-45 cells (B) and SGC-7901 (C). DAPI was used to stain nuclei. D Western Blot analysis of proteins RIP with anti-Ago2 or anti-IgG antibody in SGC-7901 and MKN-45 cells. E RNA pull-down assay using bio-NC or Bio-LINC01564 probe in SGC-7901 and MKN-45 cells. Significant differences are indicated by ***p < 0.001