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Fig. 4 | Journal of Translational Medicine

Fig. 4

From: The interaction between long non-coding RNA LINC01564 and POU2F1 promotes the proliferation and metastasis of gastric cancer

Fig. 4

LINC01564 regulated GC cell proliferation invasion and EMT. A The RNA level of LINC01564 was detected by qPCR. SGC-7901 and MKN-45 cells were transfected with the negative control (NC), si-LINC01564-69, si-LINC01564-214, si-LINC01564-506, as well as LINC01564 overexpression vector (Positive control, PC). B The RNA levels of LINC01564 (left) and POU2F1 (right) were detected by qPCR after LINC01564 overexpression and knockdown. C The protein levels of POU2F1, E-cadherin, Vimentin, and β-actin were analyzed using Western blot. D MTT assay was conducted in SGC-7901 (left) and MKN-45 (right) cells. X axis represents time, while Y axis represents cell proliferation rate. Cell proliferation rate was measured at 0 h, 12 h, 24 h, 48 h, and 72 h time points. E Cell invasion assay was conducted in SGC-7901 and MKN-45 cells transfected with NC, LINC01564 (OE), or LINC01564 (KD). F Colony formation assay was conducted in SGC-7901 and MKN-45 cells transfected with NC, LINC01564 (OE), or LINC01564 (KD). G The expression levels of E-cadherin and Vimentin were further analyzed by IF. Significant differences are indicated by **p < 0.01 and ***p < 0.001

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