Fig. 4

ANGPT2 downregulation promoted albumin transcytosis. HK-2 was transfected with shANGPT2, then the effect on albumin transcytosis was detected. A Representative immunoblot of ANGPT2 with shANGPT2 for 72 h. B Densitometric analysis of ANGPT2 immunoblot signals (n ≥ 3). ^*P < 0.05 vs. scra (scramble shRNA). C Statistical IF signals in the upper chambers of Transwell (n ≥ 3). ^**P < 0.01 vs. NG + scra, ^##P < 0.01 vs. NG + scra, ^&P < 0.05 vs. HG + scra. D Statistical immunofluorescence (IF) signals in in the lower chambers of Transwell (n ≥ 3). ^**P < 0.01 vs. NG + scra, ^## P < 0.01 vs. NG + scra, ^&&P < 0.01 vs. HG + scra