Fig. 4

circMAP3K4 is a sponge of miR-6795-5p. A The predicted binding sites of circMAP3K4 on miR-6795-5p and mutant binding sites. B The dual-luciferase reporter assay was used to confirm the interaction between circMAP3K4 and miR-6795-5p. C Fluorescence in situ hybridization showed that circMAP3K4 and miR-6795-5p were co-located in the cytoplasm of HTR-8/SVneo cells; the red color represents circMAP3K4, while the green color represents miR-6795-5p. D circMAP3K4 expression was measured by qPCR to assess the transfection efficiency of sh-circMAP3K4. E miR-6795-5p expression in the GDM (n = 42) and NGT (n = 42) groups as determined by qPCR. F miR-6795-5p expression in HTR-8/SVneo cells in the different groups as determined by qPCR. The cell experiment was repeated three times. *P < 0.05, **P < 0.01, ***P < 0.001, ns: no significant difference