Fig. 3

Identification of circMAP3K4 in GDM placentas and IR trophoblast model. A circMAP3K4 expression in the GDM (n = 42) and NGT (n = 42) groups as determined by qPCR. B, C circMAP3K4 expression in the placentas of GDM patients (n = 42) was positively correlated with weight gain during pregnancy and time-glucose AUC of the OGTT. D Insulin-PI3K/Akt signaling pathway markers were evaluated by western blotting in the GDM and NGT groups; the representative images of western blotting were displayed. E Construction of the IR model of HTR-8/SVneo cells. Glucose uptake ability was significantly decreased in IR group as compared to control cells. F circMAP3K4 expression in HTR-8/SVneo cells in the IR and control groups as determined by qPCR. G circMAP3K4 could resist digestion by RNase R, and circMAP3K4 was amplified to cDNA but not to gDNA using divergent primers. H Sanger sequence examined the circular splicing site of circ MAP3K4. I qRT-PCR analysis of circMAP3K4 in either the cytoplasm or the nucleus in HTR8/SVneo cells. The cell experiment was repeated three times. *P < 0.05, **P < 0.01, ***P < 0.001, ns: no significant difference